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Merck

MABE923

Anti-Histone H3, clone 1B1-B2 Antibody

clone 1B1-B2, from mouse

Synonym(s):

Histone H3.3C, Histone H3.5

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Histone H3, clone 1B1-B2 Antibody, clone 1B1-B2, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1B1-B2, monoclonal

species reactivity

yeast, mouse, human

species reactivity (predicted by homology)

vertebrates (based on 100% sequence homology)

technique(s)

ChIP: suitable
western blot: suitable

isotype

IgG3κ

NCBI accession no.

NP_001013721

UniProt accession no.

Q6NXT2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa cell lysate.

Application

Anti-Histone H3 antibody, clone 1B1-B2 is an antibody against Histone H3 for use in western blotting & ChIP.
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H3 from mouse embyonic fibroblasts (MEF).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.
~15 kDa observed

Immunogen

Epitope: C-terminus
Linear peptide corresponding to the C-terminus of human Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enaam Chleilat et al.
International journal of molecular sciences, 21(8) (2020-04-25)
Calcium homeostasis is a cellular process required for proper cell function and survival, maintained by the coordinated action of several transporters, among them members of the Na+/Ca2+-exchanger family, such as SLC8A3. Transforming growth factor beta (TGF-β) signaling defines neuronal development
Milena Kluge et al.
Journal of cellular physiology, 234(9), 15061-15079 (2019-01-17)
Bicarbonate concentration in saliva is controlled by the action of acid-base transporters in salivary duct cells. We show for the first time expression of ATP6V1B1 in submandibular gland and introduce transforming growth factor-beta (TGF-β) as a novel regulator of V-ATPase

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