Anti-DNA polymerase II subunit A Antibody, clone 9F11.1

DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7; UniProt Q07864; also known as DNA polymerase II subunit A) is encoded by the POLE (also known as CRCS12, FILS, POLE1) gene (Gene ID 5426) in human. DNA polymerase II is one of the six known mammalian template-directed DNA polymerases. It is composed of two subunits (261 kDa and 59 kDa). The larger catalytic subunit is a B-type polymerase family member, containing polymerase and intrinsic 3′-5′ exonuclease activities in its N-terminal part and a putative zinc-finger domain in its C-terminus. POLE gene mutations are linked to colorectal cancer, facial dysmorphism, immunodeficiency, livedo, and short stature.

~260 kDa observed

Epitope: Near N-terminus

GST-tagged recombinant protein corresponding to human DNA polymerase II subunit A near the N-terminus.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-DNA polymerase II subunit A Antibody, clone 9F11.1 is validated for use in Western Blotting for the detection of DNA polymerase II subunit A .

Clone 9F11.1 reacts with the 220 kD catalytic subunit of DNA polymerase epsilon. It does not recognize DNA polymerases alpha, beta or delta.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in human erythroleukemia K562 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DNA polymerase II subunit A in 10 µg of human erythroleukemia K562 cell lysate.

Concentration: Please refer to lot specific datasheet.

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