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Merck

MABE969

Anti-VprBP Antibody, clone 8A7.2

clone 8A7.2, from mouse, purified by using protein G

Synonym(s):

Protein VPRBP;DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-VprBP Antibody, clone 8A7.2, clone 8A7.2, from mouse, purified by using protein G

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8A7.2, monoclonal

purified by

using protein G

species reactivity

human, rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2b

NCBI accession no.

NP_055518

UniProt accession no.

Q9Y4B6

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... VPRBP(9730)

Analysis Note

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected VprBP in 10 µg of HCT116 human colon carcinoma cell lysate.

Application

Anti-VprBP Antibody, clone 8A7.2 is an antibody against VprBP for use in Western Blotting, Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected VprBP in human kidney, human small intestine, and rat testis tissue sections.
Research Category
Epigenetics
Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Expected to react with all three spliced isoforms of human VprBP reported by UniProt (Q9Y4B6).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein VPRBP (EC 2.7.11.1; UniProt Q9Y4B6; also known as DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP) is encoded by the VPRBP (also known as DCAF1, KIAA0800, RIP) gene (Gene ID 9730) in human. VprBP/DCAF1 acts as an adaptor molecule that mediates substrates recruitment for both the RING-type CRL4 (Cul4-Roc1-DDB1) and HECT-type EDD/UBR5 E3 ubiquitin ligase complexes. CRL4 substrates known to be recruited by VprBP include Merlin, Mcm10, UNG2, SMUG1, RORαme1. Known EDD/UBR5 substrates recruited by VprBP include Katanin and TERT. VprBP is involved in a variety of normal cellular processes, including proliferation, DNA replication, cell cycle progression, telomere maintenance, and DNA damage responses. As indicated by its name, VprBP is also a well known target utilized by the Vpr and Vpx accessory factors of primate lentiviruses to highjack the host CRL4VprBP E3 ligase function.
~169 kDa observed. 169.0 kDa (isoform 1), 168.9 kDa (isoform 2), and 118.2 kDa (isoform 3) calculated.

Immunogen

BCP-conjugated linear peptide corresponding an N-terminal sequence of human VprBP.
Epitope: N-terminal region.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABE96904055977331233

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