MABE982

Anti-RBP-Jk, clone 1F1 Antibody

Name: Anti-RBP-Jk, clone 1F1 Antibody
Brand: MM

clone 1F1, from rat

Synonym(s):

Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, RBP-J, RBP-JK, Renal carcinoma antigen NY-REN-30

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-RBP-Jk, clone 1F1 Antibody, clone 1F1, from rat

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1F1, monoclonal

species reactivity

Drosophila, human

technique(s)

ChIP: suitable
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

NCBI accession no.

NP_005340

UniProt accession no.

Q06330

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... RBPJ(3516)

Application

This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line.

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSC′s (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Analysis Note

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus.

~55 kDa observed

Immunogen

Recombinant protein corresponding to human RBP-Jk.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Protein G Purified

Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c