MABF249

Anti-Tapasin Antibody, clone 7F6

Name: Anti-Tapasin Antibody, clone 7F6
Brand: MM

clone 7F6, from rat

Synonym(s):

Tapasin, TPN, TSPN, NGS-17, TAP-associated protein, TAP-binding protein

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-Tapasin Antibody, clone 7F6, clone 7F6, from rat

biological source

rat

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

7F6, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

np_003181

UniProt accession no.

O15533

shipped in

wet ice

target post-translational modification

unmodified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Tapasin in 10 µg of HeLa cell lysate.

Application

Detect Tapasin using this rat monoclonal antibody, Anti-Tapasin Antibody, clone 7F6 validated for use in western blotting & Flow Cytometry.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Tapasin in 10 µg of A431, HEK293, HepG2, HUVEC, Jurkat, and PC12 cell lysates.

Flow Cytometry Analysis: 4 µg from a representative lot detected Tapasin in 1X10E6 PBMC cells.

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA transfected MJS cell lysate (Hislop, A. D., et al. (2007). J Exp Med. 204(8):1863-1873.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in HeLa cell lysate (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in BNLF2aHA4M transfected MJS cell lines (Horst, D., et al. (2009). J Immunol. 182(4):2313-2314.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Tapasin in WB (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

Biochem/physiol Actions

This antibody recognizes the Lumenal domain of Tapasin.

General description

Tapasin (TPN or TPSN) is also known as NGS-17, TAP-associated protein and TAP-binding protein. Tapasin plays a role in MHC class I peptide loading and assembly, and is involved in associating MHC class I with transporter associated with antigen processing (TAP). Tapasin is found in neutrophils, predominantly within fully differentiated cells.

~48 kDa observed

Immunogen

Linear peptide corresponding to human Tapasin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Assembly of the MHC I peptide-loading complex determined by a conserved ionic lock-switch.

Andreas Blees et al.

Scientific reports, 5, 17341-17341 (2015-11-28)

Salt bridges in lipid bilayers play a decisive role in the dynamic assembly and downstream signaling of the natural killer and T-cell receptors. Here, we describe the identification of an inter-subunit salt bridge in the membrane within yet another key

Global Trade Item Number

SKU	GTIN
MABF249	04053252911927

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