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Merck

MABF264

Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody

clone 13F10, from rat

Synonym(s):

WD repeat-containing protein 1, Actin-interacting protein 1, AIP1, AIP1/WD repeat-containing protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
13F10, monoclonal
Application:
WB
Citations:
1

Key Documents

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biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

13F10, monoclonal

species reactivity

mouse

technique(s)

western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_035845

UniProt accession no.

O88342

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

300

Gene Information

mouse ... Wdr1(22388)

General description

Actin-interacting protein 1 (AIP1), also known as WD repeat-containing protein 1 (Wdr1), is a key player in cytoskeletal rearrangements and actin mobilization, and thus plays a role in many cellular situations from movement to cytokinesis. AIP1 interacts with Cofilin as well, and Cofilin regulates actin filament dynamics by stimulating actin filament disassembly and also plays a critical role in cytokinesis and chemotactic migration. Knockdown of AIP1 in HeLa cells increased the percentage of multinucleate cells and the mechanics of cytokinesis were altered with an excessive buildup of actin filaments at telophase. The results could be relieved by overexpression of Cofilin pointing to AIP1’s critical role in Cofilin-mediated actin filament disassembly. Furthermore, AIP1 inhibition caused abnormal cell migration in response to cytokine stimulation leading to a non-directional migration phenotype thus indicating that AIP1 plays an important directional role in response to stimuli as well. Finally AIP1, as one might expect, plays a critical role in development of cells and tissues, especially in epithelial morphogenesis for instance.
~66 kDa observed

Immunogen

Recombinant protein corresponding to mouse AIP1/WD repeat-containing protein 1.

Application

This Anti-AIP1/WD repeat-containing protein 1, clone 13F10 Antibody is validated for use in Western Blotting for the detection of AIP1/WD repeat-containing protein 1.
Western Blotting Analysis: A representative lot detected AIP1/WD repeat-containing protein 1 in both endogenous and transfected mouse AIP1 in western blots of cell lysates from J774 cells, 293T cells and 293T cells transfected with HA-tagged mouse Aip1 (Li, J., et al. (2007). Nature. 9(3):276-286).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected AIP1/WD repeat-containing protein 1 in 10 µg of RAW264.7 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABF26404053252993145

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