MABF293

Anti-CD46 Antibody, clone 8A9.1

Name: Anti-CD46 Antibody, clone 8A9.1
Brand: MM

clone 8A9.1, from mouse

Synonym(s):

Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, CD46

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8A9.1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_002380

UniProt accession no.

P15529

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD46(4179)

Related Categories

Flow Cytometry Antibodies

Protein Biology

General description

43.7 kDa (isoforms A; no del ABC1) and 44.2 kDa (isoforms B; del 13 ABC2) calculated.

Membrane cofactor protein (UniProt P15529; also known as Antigen identified by monoclonal antibody TRA-2-10, CD46 antigen, Complement membrane cofactor protein, Measles virus receptor membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, Trophoblast-lymphocyte cross-reactive antigen) is encoded by the CD46 (also known as MCP, TLX, AHUS2, MIC10, TRA2.10) gene (Gene ID 4179) in human. Signal 1-34, Membrane cofactor protein (MCP) or CD46 is a widely expressed complement regulator that inhibits all three pathways of complement activation, classical (CP), alternative (AP), and lectin (LP). It binds to both C3b (AP) and C4b (CP) and acts as a cofactor for their inactivation by the C3b/C4b inactivator enzyme factor I. CD46 is initially produced with an N-terminal signal sequence (a.a. 1-34), which is post-translationally removed to yield the type 1 membrane bound CD46 consisting of a large extracellular (a.a. 35-343) portion, followed by a single transmembrane α-helical region (a.a. 344-366) and a cytoplasmic tail (a.a. 367-392) with several variant structures. The extracellular portion is composed of four extracellular Complement Control Protein (CCP) domains carrying the cofactor activity, a collar STP-rich region, and a short region of unknown function (U). CD46 also acts as a receptor for certain pathogens such as the measles virus (Edmonston strain), human herpesvirus 6, and Neisseria gonorrhoeae. CD46 is also implicated to have a role in human egg fertilization. Several mutations in the CCPs and the transmembrane region of CD46 are linked to atypical hemolytic uremic syndrome (aHUS).

Immunogen

mIg Fc-tagged recombinant protein corresponding to the extracellular domain of human CD46.

Application

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected CD46 in HeLa and A431 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD46 in 1X10E6 HeLa cells.

This Anti-CD46 Antibody, clone 8A9.1 is validated for use in Western Blotting, Immunocytochemistry and Flow Cytometry for the detection of CD46.

Biochem/physiol Actions

Expected to react with isoforms A (no del, ABC1) and isoform B (del 13, ABC2). Reactivity toward other spliced isoforms (Uniprot P15529-3 through P15529-16) has not been determined.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in human CD46 extracellular domain mIg Fc fusion protein.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CD46 in 0.25 µg of human CD46 extracellular domain mIg Fc fusion protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKU	GTIN
MABF293	04055977294330

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