Key Documents

COA/COQ

MABF33

Anti-MHC class II (I-A/I-E) Antibody, clone M5/114

Name: Anti-MHC class II (I-A/I-E) Antibody, clone M5/114
Brand: MM

clone M5/114, from rat

Synonym(s):

CD74 molecule, major histocompatibility complex, class II invariant chain, Ia-associated invariant chain, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), gamma chain of class II antigens, Ia antigen-

Sign Into View Organizational & Contract Pricing

All Photos(1)

About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

M5/114, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001036070

UniProt accession no.

P04441

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Cd74(16149)

General description

MHC class II-associated invariant chain (I-A/I-E), also known as Ia antigen-associated invariant chain, are single-pass type II membrane glycoproteins containing a single thyroglobulin type-I domain. They are critical components in the processing of MHC class II antigen through forming a stabilizing complex following the alpha/beta heterodimer synthesis, and orchestrating complex trafficking away from the ER compartments in which class II peptide loading occurs.

~32 kDa observed.
There are 2 isoforms produced by alternative splicing: 32 kDa Isoform Long and 24 kDa Isoform Short.

Application

Detect MHC class II (I-A/I-E) using this Anti-MHC class II (I-A/I-E) Antibody, clone M5/114 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC on spleen cells. (Bhattacharya, A., et al. (1981). The Journal of immunology. 127(6):2488-2496.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH on thymus tissue. (de Bont, E., et al. (1999). Int. Immunol. 11(8):1295-1306.)

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MHC class II (I-A/I-E) in 10 µg of mouse spleen tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Limitations of Qdot labelling compared to directly-conjugated probes for single particle tracking of B cell receptor mobility.

Libin Abraham et al.

Scientific reports, 7(1), 11379-11379 (2017-09-14)

Single-particle tracking (SPT) is a powerful method for exploring single-molecule dynamics in living cells with nanoscale spatiotemporal resolution. Photostability and bright fluorescence make quantum dots (Qdots) a popular choice for SPT. However, their large size could potentially alter the mobility

Circulating Porphyromonas gingivalis lipopolysaccharide resets cardiac homeostasis in mice through a matrix metalloproteinase-9-dependent mechanism.

Deleon-Pennell, KY; de Castro Bras, LE; Lindsey, ML

Physiological Reports null

Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation.

Madison Bolger-Munro et al.

eLife, 8 (2019-06-04)

When B cells encounter antigens on the surface of an antigen-presenting cell (APC), B cell receptors (BCRs) are gathered into microclusters that recruit signaling enzymes. These microclusters then move centripetally and coalesce into the central supramolecular activation cluster of an

Dark microglia: A new phenotype predominantly associated with pathological states.

Kanchan Bisht et al.

Glia, 64(5), 826-839 (2016-02-06)

The past decade has witnessed a revolution in our understanding of microglia. These immune cells were shown to actively remodel neuronal circuits, leading to propose new pathogenic mechanisms. To study microglial implication in the loss of synapses, the best pathological

Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo.

Mahmoud Elashiry et al.

Journal of extracellular vesicles, 9(1), 1795362-1795362 (2020-09-19)

Chronic bone degenerative diseases represent a major threat to the health and well-being of the population, particularly those with advanced age. This study isolated exosomes (EXO), natural nano-particles, from dendritic cells, the "directors" of the immune response, to examine the

View All Related Papers

Global Trade Item Number

SKU	GTIN
MABF33	04053252413872

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service