MABF508

Anti-CD115 (c-fms) Antibody (mouse), PE, clone AFS98

Name: Anti-CD115 (c-fms) Antibody (mouse), PE, clone AFS98
Brand: MM

clone AFS98, 0.2 mg/mL, from rat

Synonym(s):

Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms, CD115, CSF1-R

Select a Size

All Photos(1)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-CD115 (c-fms) Antibody (mouse), PE, clone AFS98, clone AFS98, 0.2 mg/mL, from rat

biological source

rat

conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

AFS98, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG2aκ

UniProt accession no.

P09581

shipped in

wet ice

target post-translational modification

unmodified

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 µg of this antibody detected CD115 (c-fms) in one million C57Bl/6 peripheral blood cells.

Application

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-CD115 (c-fms) (mouse), PE, clone AFS98 validated for use in western blotting & Flow Cytometry for the detection of CD115 (c-fms).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor.

Immunogen

Immunogen information for this antibody is proprietary information.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Not finding the right product?

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Global Trade Item Number

SKU	GTIN
MABF508	04053252979330

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service