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Merck

MABF592

Anti-CD45.2 Antibody (mouse), violetFluor® 450, clone 104

clone 104, 0.2 mg/mL, violetFluor®

Synonym(s):

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-CD45.2 Antibody (mouse), violetFluor® 450, clone 104, clone 104, 0.2 mg/mL, violetFluor®

biological source

mouse

conjugate

violetFluor® 450

antibody form

purified antibody

antibody product type

primary antibodies

clone

104, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

violetFluor®

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2aκ

UniProt accession no.

P06800

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

mouse ... Ptprc(19264)

Related Categories

Legal Information

violetFluor is a registered trademark of Tonbo Biotechnologies

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45.2 in one million C57Bl/6 splenocytes.

Application

Research Category
Inflammation & Immunology
This mouse monoclonal antibody Anti-CD45.2 (mouse), violetFluor 450, clone 104 validated for use in IP, Flow Cytometry & ICC for the detection of CD45.2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to specific for CD45.2 and is not cross-reactive with CD45.1-bearing cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

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Storage Class

12 - Non Combustible Liquids

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nwg

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABF59204053252963988

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