Anti-Myeloperoxidase Antibody, clone CLB-MPO-1/1,7.17, FITC conjugate

Myeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of neutrophils.

~73/83/87 kDa calculated

Purified human MPO.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-Myeloperoxidase Antibody, clone CLB-MPO-1/1,7.17, FITC conjugate is validated for use in Flow Cytometry for the detection of Myeloperoxidase.

This monoclonal antibody reacts with majority cell types of the myeloid lineage, including myeloblasts, neutrophils, and monocytes. It does not react with human lymphocytes, thrombocytes, or erythrocytes. 96% of all acute myelogenous leukemias (AMLs) are found positive, 100% of all acute lymphoblastic leukemias (ALLs) are found negative when stained with this monoclonal antibody.

Purified by Ion-Exchange Chromatography

Purified mouse conjugate IgG2aκ monoclonal antibody in buffer containing 20mM Tris, 150mM NaCl, pH8.0 with 1% BSA (w/v), and 0.1% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 0.01 mL of this antibody detected Myeloperoxidase in human peripheral blood monocytes (PBMCs).

Concentration: Please refer to lot specific datasheet.

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