Key Documents
MABN153
Anti-HuC/HuD Antibody, clone 15A7.1
clone 15A7.1, from mouse
Synonym(s):
ELAV-like protein 3, Hu-antigen C, HuC, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, ELAV-like protein 4, Hu-antigen D, HuD, Paraneoplastic encephalomyelitis antigen HuD
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
15A7.1, monoclonal
species reactivity
human, rat, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ELAVL4(1996)
General description
Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding proteins that contain three RNA recognition motifs. RNA binding protein HuC promotes neuronal development in both the central and the peripheral nervous systems. HuC antibodies are used as markers for developing neurons. HuD may play a role in neuron-specific RNA processing, and be involved in maintaining the proliferative state of cells.
~ 40 kDa observed. An uncharacterized band may appear at ~45 kDa in some lysates.
Immunogen
KLH-conjugated linear peptide corresponding to human HuC/HuD.
Application
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected HuC/HuD in human brain and rat hippocampus tissues.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This HuC/HuD antibody is validated for use in WB & IHC for the detection of the HuC/HuD protein.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human brain tissue lysate
Human brain tissue lysate
Evaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected HuC/HuD in 200 µg of human brain tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Christine Marques et al.
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Karolína Liška et al.
Neuroendocrinology, 112(4), 384-398 (2021-06-11)
Circadian clocks in the hippocampus (HPC) align memory processing with appropriate time of day. Our study was aimed at ascertaining the specificity of glycogen synthase kinase 3-beta (GSK3β)- and glucocorticoid (GC)-dependent pathways in the entrainment of clocks in individual HPC
Yaxing Gui et al.
Brain pathology (Zurich, Switzerland), 30(1), 151-164 (2019-07-06)
The 18 kDa translocator protein (TSPO) is a widely used target for microglial PET imaging radioligands, but its expression in post-mortem normal and diseased human brain is not well described. We aimed at characterizing the TSPO expression in human control
Clara Muñoz-Castro et al.
Methods in molecular biology (Clifton, N.J.), 2593, 283-305 (2022-12-14)
There is a growing interest in expanding the multiplexing capability of immunohistochemistry to achieve a deeper phenotyping of various cell types in health and disease. Here, we describe a protocol of cyclic multiplex fluorescent immunohistochemistry that enables the labeling of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABN153 | 04053252858307 |
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