MABN1787

Anti-NAV1 Antibody, clone 7F12 (KT124)

Name: Anti-NAV1 Antibody, clone 7F12 (KT124)
Brand: MM

clone KT124(7F12), from rat

Synonym(s):

Neuron navigator 1, Pore membrane and/or filament-interacting-like protein 3, Steerin-1, Unc-53 homolog 1, unc53H1, NAV1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

KT124(7F12), monoclonal

species reactivity

mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001161210

UniProt accession no.

Q8NEY1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NAV1(89796)

General description

Neuron navigator 1 (UniProt Q8NEY1; also known as Pore membrane and/or filament-interacting-like protein 3, Steerin-1, Unc-53 homolog 1, unc53H1) is encoded by the NAV1 (also known as KIAA1151, KIAA1213, POMFIL3, STEERIN1, UNC53H1) gene (Gene ID 89796) in human. NAV1 is a member of the family of proteins called navigators that play a role in mediating neurite outgrowth. NAV1 is shown to interact with the guanine nucleotide exchange factor (GEF) TRIO, whereby it enhances the affinity of TRIO for Rho family GTPases Rac1 and RhoG. The plus-end-tracking protein (+TIP) EB1 at the ends of growing microtubules recruits TRIO, facilitating the formation and localization of functional NAV1-TRIO complexes, which in turn regulates neurite outgrowth by selectively activating Rac1 at the growth cone.

~240/150 kDa observed. 202.5 kDa (isoform 1), 201.5 kDa (isoform 2), 202.1 kDa (isoform 3), 196.0 kDa (isoform 4), 160.8 kDa (isoform 5), 126.2 kDa (isoform 6), 195.0 kDa (isoform 7) calculated.

Immunogen

His-tagged recombinant protein corresponding to the Internal region of human NAV1.

Application

Anti-NAV1 Antibody, clone 7F12 (KT124) is an antibody against NAV1 for use in Immunocytochemistry, Western Blotting.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected NAV1 in 10 µg of Murine NIE-115 neuroblastoma cell lysate.
Immunocytochemistry Analysis: 10.0 µg/mL from a representative lot detected NAV1 in Neuro-2a and NIE-115 cells.
Western Blotting Analysis: A representative lot detected endogenous NAV1 protein in murine neuroblastoma N1E-115 cell lysate, as well as exogenously expressed NAV1, but not NAV2 or NAV3, GFP fusion protein in lysates from transfected HEK293T cells (van Haren, J., et al. (2014). Curr. Biol. 24(15):1778-1785).
Immunocytochemistry Analysis: A representative lot detected NAV1 accumulation in cellular extensions of primary murine hippocampal neurons and murine neuroblastoma N1E-115 cells (van Haren, J., et al. (2014). Curr. Biol. 24(15):1778-1785).

Biochem/physiol Actions

Expected to react with all seven spliced isoforms of murine NAV1 (UniProt Q8NEY1-1 through Q8NEY1-7), but not NAV2 or NAV3.

Physical form

Format: Purified

Purified rat monoclonal IgG2aκ antibody in PBS and 0.09% sodium azide.

Analysis Note

Evaluated by Western Blotting in murine Neuro-2a (N2a) neuroblastoma cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NAV1 in 10 µg of murine Neuro-2a (N2a) neuroblastoma cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Certificates of Analysis (COA)

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Global Trade Item Number

SKU	GTIN
MABN1787	04055977297690

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