MABN385

Anti-NHERF-2 Antibody, clone 32B6

Name: Anti-NHERF-2 Antibody, clone 32B6
Brand: MM

clone 32B6, from mouse

Synonym(s):

Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHERF-2, NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1, SIP-1, Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2, Tyrosine kinase ac

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-NHERF-2 Antibody, clone 32B6, clone 32B6, from mouse

antibody product type

primary antibodies

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

32B6, monoclonal

species reactivity

rat, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_001123484

UniProt accession no.

Q15599

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SLC9A3R2(9351)

Analysis Note

Control
HaCaT keratinocytes cell lysate

Evaluated by Western Blotting in HaCaT keratinocytes cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NHERF-2 in 10 µg of HaCaT keratinocytes cell lysate.

Application

Anti-NHERF-2 Antibody, clone 32B6 is a highly specific mouse monoclonal antibody, that targets NHERF2 & has been tested in western blotting, ICC & IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in A431 and NHERF-2 transfected HT1080 cells. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Batt cell lysate. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) is also known as NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1 (SIP-1), Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2 (SLC9A3R2), and Tyrosine kinase activator protein 1 (TKA-1). NHERF-2 is a Scaffold protein that links ezrin/moesin/radixin family members to the actin cytoskeleton and regulates their surface expression. NHERF-2 is critical for the cAMP-mediated phosphorylation and inhibition of SLC9A3. NHERF-2 is also thought to act as scaffold protein in the nucleus.

~ 37 kDa observed. An uncharacterized band may appear at ~41 kDa in some lysates.

Immunogen

recombinant protein corresponding to human NHERF-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c