Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1

The protein named Mitochondrial dicarboxylate carrier or Solute carrier family 25 member 10, or Dicarboxylate Carrier Slc25a10 and encoded by the gene named SLC25A10 or DIC is a mitochondrial transport protein that mediates the transfer of malonate, malate, and succinate in exchange for phosphate, sulfate, sulfite or thiosulfate across the mitochondrial inner membrane. These components are critical for the function of the Krebs cycle, as well as gluconeogenesis, urea and sulfur metabolism. Mitochondrial dicarboxylate carrier is expressed in high amounts in the liver and kidney and at lower levels in all other tissues and is localized to the mitochondria and specifically to the inner mitochondrial membrane. Mitochondrial dicarboxylate carrier is also upregulated in various tissues in response to free fatty acids particularly in adipose tissues, and plays a role in mitochondrial malate transport and in the regulation of glucose-stimulated insulin secretion from pancreatic beta cells. Impairment of Mitochondrial dicarboxylate carrier is associated with ethylmalonic encephalopathy (EE) and short chain acyl-CoA dehydrogenase deficiency (SCADD) syndromes. Research indicates that it is the buildup of ethylmalonic acid in the tissues that impairs the Succinate and malate uptake by Mitochondrial dicarboxylate carrier leading to metabolic distress and dysfunction.

~32 kDa observed

KLH-conjugated linear peptide corresponding to human Mitochondrial dicarboxylate carrier.

Anti-Mitochondrial dicarboxylate carrier Antibody, clone 1F5.1 is a highly specific mouse monoclonal antibody, that targets Solute carrier family 25 member 10 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Mitochondrial dicarboxylate carrier in human kidney and human liver tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Mitochondrial dicarboxylate carrier in 200 µg of mouse liver tissue lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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