Key Documents

COA/COQ

MABN478

Anti-Spred-1 Antibody, clone M23-P2G3

Name: Anti-Spred-1 Antibody, clone M23-P2G3
Brand: MM

clone M23-P2G3, from mouse, purified by affinity chromatography

Synonym(s):

Sprouty-related, EVH1 domain-containing protein 1, Spred-1, hSpred1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

M23-P2G3, monoclonal

purified by

affinity chromatography

species reactivity

rat, mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1λ

NCBI accession no.

NP_689807

UniProt accession no.

Q7Z699

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SPRED1(161742)

General description

Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus. SPRED1 associated with Ras blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes. A mutation in SPRED1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome.

~ 60 kDa observed

Immunogen

Linear peptide corresponding to human Spred-1.

Application

Detect Spred-1 using this mouse monoclonal antibody, Anti-Spred-1 Antibody, clone M23-P2G3 validated for use in western blotting & ICC.

Immunocytochemistry Analysis: A representative lot detected Spred-1 in HeLa cells.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Physical form

Protein G Purified

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Spred-1 in 10 µg of mouse brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)