MABS1195

Anti-SSH1 Antibody, clone 12C6.1

Name: Anti-SSH1 Antibody, clone 12C6.1
Brand: MM

clone 12C6.1, from mouse

Synonym(s):

Protein phosphatase Slingshot homolog 1, hSSH-1L, Slingshot homolog 1, SSH-1L, SSH-like protein 1

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

12C6.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_061857

UniProt accession no.

Q8WYL5

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SSH1(54434)

General description

Protein phosphatase Slingshot homolog 1 (EC 3.1.3.16; EC 3.1.3.48; UniProt Q8WYL5; also known as hSSH-1L, Slingshot homolog 1, SSH-1L, SSH-like protein 1) is encoded by the SSH1 (also known as KIAA1298, SSH1L) gene (Gene ID 54434) in human. SSH1 is the founding member of the Slingshot family of protein phosphatases that function to destabilize the actin cytoskeleton by reversing cofilin Ser3 phosphorylation and thereby re-activating cofilin. Cofilin plays a central role in regulating actin filament turnover and actin cytoskeletal remodelling by severing and depolymerizing actin filaments. Cofilin activity is negatively regulated by LIM kinases-catalyzed Ser3 phosphorylation. The Slingshot family members include SSH-1L, SSH-2L, SSH-3L, and chronophin. The members differ in their subcellular distribution, F-actin-binding activity, and phosphatase activity, implying related but member-specific functions.

~150 kDa observed. 115.5 kDa (isoform 1), 77.43 kDa (isoform 2), 80.68 kDa (isoform 4), and 78.98 kDa (isoform 5) calculated.

Immunogen

GST-tagged recombinant protein corresponding to an internal sequence of human SSH1.

Application

Anti-SSH1 Antibody, clone 12C6.1 is an antibody against SSH1 for use in Western Blotting.

Biochem/physiol Actions

Expected to react with spliced isoform 1 (isoform L), isoform 2 (isoform S), isoform 4, and isoform 5, but not isoform 3 (isoform B) reported by UniProt (Q8WYL5).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SSH1 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)