Anti- LTP/CETP Antibody, clone 3-11D

Cholesteryl ester transfer protein (UniProt P22687; also known as CETP, Lipid transfer protein I, LTP) is encoded by the CETP (also known as HDLCQ10) gene (Gene ID 1071) in Oryctolagus cuniculus (Rabbit) species. Cholesteryl ester transfer protein (CETP) or lipid transfer protein (LTP) mediates the transport/exhanges of triglycerides and cholesteryl esters (CEs) between very-low-density lipoproteins (VLDL)/low-density lipoproteins (LDL) and high-density lipoproteins (HDL) in both directions. CETP plays an important role in the reverse cholesterol transport (RCT) pathway that mediates the transport of cholesterol from peripheral cells and tissues to the liver for metabolism and excretion in the bile. CETP shuttles CEs from HDL particles to apolipoprotein B-/apoB-containing particles in exchange for triglycerides. CETP is thought to facilitate the generation of small, dense LDL (sd LDL) particles through an indirect mechanism of increased rate of triglyceride transfer from VLDL in exchange for CEs in LDL and HDL. CE transfer between lipoproteins plays a crucial role in intravascular LDL remodeling. Decreased levels of HDL cholesterol and elevated levels of LDL is a risk factor for atherosclerosis and coronary heart disease (CHD).

~70 kDa observed. Target band appears larger than the calculated molecular weight of 54.5 kDa due to glycosylation.

Native rabbit plasma CETP/LTP.

Anti- LTP/CETP Antibody, clone 3-11D is an antibody against LTP/CETP for use in Western Blotting, ELISA, Inhibits Activity/Function.

ELISA Analysis: Representative lots were used as the capture antibody in combination with clone 14–8F as the detection antibody for the detection of human plasma CETP/LTP by sandwich ELISA (Tosheska, K., et al. (2011). Bosn. J. Basic. Med. Sci. 11(3):169–173; Saito, K., et al. J. Lipid Res. (1999). 40(11):2013-2021; Sasai, K., et al. (1998). Clin. Chem. 44(7):1466-1473).
ELISA Analysis: A representative lot was used as the capture antibody in combination with clone 14–8F as the detection antibody for the detection of mouse plasma CETP/LTP by sandwich ELISA (Wu, C.A., et al. (2002). Arterioscler .Thromb. Vasc. Biol. 22(8):1347-1353).
ELISA Analysis: A representative lot showed no significant pH-dependent CETP/LTP-capturing activity in the pH range from 5.0 to 7.5 in sandwich ELISA applications (Saito, K., et al. J. Lipid Res. (1999). 40(11):2013-2021).
Western Blotting Analysis: A representative lot detected only non-reduced, but not reduced, rabbit and human CETP/LTP by Western blotting (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot inhibited both rabbit and human CETP/LTP-mediated cholesteryl ester (CE) and triglyceride (TG) transfer from low density lipoprotein (LDL) to high density lipoprotein (HDL) (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot blocked the lipid association of both rabbit or human CETP/LTP with microemulsion (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot inhibited human CETP/LTP-mediated cholesteryl ester (CE) and triglyceride (TG) transfer from high density lipoprotein (HDL) to to apoB-containing lipoproteins in human plasma (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).

Research Category
Signaling

Research Sub Category
Lipid Metabolism & Weight Regulation

Clone 3-11D (a.k.a. 3-9F) reacts with non-reduced, but not reduced, LTP/CETP (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting of purified human plasma CETP/LTP.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected purified human plasma CETP/LTP under non-reducing condition.

Concentration: Please refer to lot specific datasheet.

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