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Merck

MABS1257

Anti-APC4 Antibody, clone CIV1.1

clone CIV1.1, from mouse

Synonym(s):

Anaphase-promoting complex subunit 4, APC4, Cyclosome subunit 4

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About This Item

eCl@ss:
32160702
UNSPSC Code:
12352203

Key Documents

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biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CIV1.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

NP_037499

UniProt accession no.

Q9UJX5

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ANAPC4(29945)

General description

92.12/92.22 kDa (isoform 1/3) calculated.
Anaphase-promoting complex subunit 4 (UniProt Q9UJX5; also known as APC4, Cyclosome subunit 4) is encoded by the ANAPC4 (also known as APC4) gene (Gene ID 29945) in human. APC4 is a component of the anaphase-promoting complex or cyclosome (APC/C). APC/C is an E3 ubiquitin ligase complex that promotes the transition from metaphase to anaphase of the cell cycle by targeting specific proteins for proteasomal degradation at specific times under the control of the spindle assembly checkpoint (SAC). The catalytic core of the APC/C consists of the cullin subunit APC2 and RING H2 domain subunit APC11. Other APC/C components, including APC4, are composed multiple repeat motifs with the main purpose of providing molecular scaffold support. APC4 contains WD repeats and has a molecular weight of approximately 92 kDa. APC4 is located in the nucleus during interphase and at the centrosome during metaphase/anaphase.

Immunogen

Linear peptide corresponding to human APC4 C-terminal end sequence.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected APC4 in human spleen tissue section.

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Cdc20, Mad2, and BubR1 with APC4 from prometaphase HeLa cells (Di Fiore, B., et al. (2015). Dev. Cell. 32(3):358-372).

Western Blotting Analysis: A representative lot detected APC4 in whole cell lysates, as well as in FLAG-tagged cyclin A and FLAG-tagged BubR1 immunoprecipitates from prometaphase HeLa cells (Di Fiore, B., et al. (2015). Dev. Cell. 32(3):358-372).
Anti-APC4, clone CIV1.1 Antibody, Cat. No. MABS1257, is a highly specific mouse monoclonal antibody that targets APC4 and has been tested in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Biochem/physiol Actions

Clone CIV1.1 target human APC4 C-terminal end sequence present in spliced isoforms 1 and 3, but absent in isoform 2 reported by UniProt (Q9UJX5).

Physical form

Format: Purified

Analysis Note

Evaluated by Immunohistochemistry in human uterus tissue section.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected APC4 in human uterus tissue section.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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