MABS1916
Anti-c-Met Antibody, clone seeMet 13
clone seeMet 13, from mouse
Synonym(s):
Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
seeMet 13, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HGF(3082)
Related Categories
General description
Immunogen
Application
Flow Cytometry Analysis: A representative lot immunostained live SNU-5 cells. A slight decrease in antibody immunoreactivity was observed when the temperature was dropped from 37°C to 4°C (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
Immunocytochemistry Analysis: A representative lot immunostained live SNU-5 cells. Indirect fluorescence labelling following subsequent cell fixation and permeabilization revealed increased antibody cytoplasmic internalization when antibody incubation was performed at 37°C than at 4°C (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
Immunoprecipitation Analysis: A representative lot immunoprecipitated c-Met from SNU-5 cell lysate (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
Inhibition Analysis: A representative lot prevented HGF-induced scatter of HaCaT cells. Clone seeMet 13 inhibited cell proliferation by blocking cell division without inducing apoptosis (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
Note: seeMet 13 exhibited low reactivity and specificity towards denatured c-Met by Western blotting. This monoclonal antibody is not recommended for Western blotting application (Wong, J.S., et al. (2013). Oncotarget. 4(7):1019-1036).
Signaling
Biochem/physiol Actions
Physical form
Preparation Note
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected both surface and internalized c-Met by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized SNU-5 cells.
Other Notes
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Global Trade Item Number
SKU | GTIN |
---|---|
MABS1916 | 04054839090233 |
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