Anti-GLEPP1 Antibody, extracellular domain, clone 1B4

138 kDa calculated

YGlomerular epithelial protein 1 (GLEPP1) is a membrane bound tyrosine phosphatase present on the apical cell surface that plays a role in regulating the glomerular pressure filtration rate by regulating podocyte structure and function. GLEPP1 is expressed in the kidney but also in brain, lung and placenta. In the brain, GLEPP1 plays an important role in axon guidance, and in other cells GLEPP1 assists in lamellipodium assembly. Because of its action on lamellipodia, GLEPP1 is important in cellular chemotaxis, thus inhibitors to GLEPP1 function aid in treatments where the migration of inflammatory cells are affected such as in ulcerative colitis. Finally, because phosphorylation and its reversal via phosphatases are key signaling and regulatory events, a variety of syndromes are associated with genetic variations associated with phosphatase mutations. For GLEPP1, various nephrotic syndromes such as NPHS6 and hyper-proteinuria disease are associated with mutations in the GLEPP1 coding region.

Recombinant protein corresponding to rat GLEPP1, extracellular domain.

Immunohistochemistry Analysis: A representative lot detected GLEPP1, extracellular domain in isolated rat glomeruli tissue (Wharram, B.L., et al. (2005) J Am Soc Nephrol. 16:2941–2952; Fukuda, A., et al. (2012). Kidney International. 81:40-55; Kim, Y.H., (2001). Kidney International. 60:957-968).
Western Blotting Analysis: A representative lot detected GLEPP1, extracellular domain in rat isolated glomeruli tissue lysate (Fukuda, A., et al. (2012). J Am Soc Nephrol. 23:1351–1363).

This Anti-GLEPP1 Antibody, extracellular domain, clone 1B4 is validated for use in Immunohistochemistry and Western Blotting for the detection of GLEPP1, extracellular domain.

Format: Purified

Evaluated by Immunohistochemistry in glomeruli of rat kidney tissue.

Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody detected GLEPP1, extracellular domain in glomeruli of rat kidney tissue.