MABS478

Anti-Nucleobindin-2 Antibody, clone 11B3.1

Name: Anti-Nucleobindin-2 Antibody, clone 11B3.1
Brand: MM

clone 11B3.1, from mouse

Synonym(s):

Nucleobindin-2, DNA-binding protein NEFA, Gastric cancer antigen Zg4, Prepronesfatin, Nesfatin-1, Nucleobindin-2

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

11B3.1, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_005004

UniProt accession no.

P80303

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NUCB2(4925)

General description

Nucleobindin-2 (UniProt P80303; also known as DNA-binding protein NEFA, Epididymis secretory protein Li 109, Gastric cancer antigen Zg4, Nucleobinding 2, Prepronefastin, Prepronesfatin) is encoded by the NUCB2 gene (also known as HEL-S-109, NEFA) (Gene ID 4925) in human. Nucleobindin-2 is initially translated as a 420 aa precursor protein. Cleavage of signal peptide (aa1-24) yields the 396 aa mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (>85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobinin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. NUCB2 is expressed in key hypothalamic nuclei and plays a role in energy homeostasis. It is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissue, suggesting its roles in various metabolic functions. NUCB2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues.

~ 50 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human Nucleobindin-2.

Application

Detect Nucleobindin-2 using this Anti-Nucleobindin-2 Antibody, clone 11B3.1 validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Nucleobindin-2 in human normal colon, human breast cancer tumor cells, rat small intestine, and mouse hippocampus tissue.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

Detects nucleobindin-2, but not nesfatin-1. Reactivity toward nesfatin-2 or nesfatin-3 has not been determined.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nucleobindin-2 in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

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