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MABT121

Sigma-Aldrich

Anti-Vimentin Antibody, clone 3CB2

clone 3CB2, from mouse

Synonym(s):

Vimentin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3CB2, monoclonal

species reactivity

chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard

species reactivity (predicted by homology)

chicken (immunogen homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

NP_001041541

UniProt accession no.

P09654

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

General description

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.
~54 kDa observed

Immunogen

Vimentin extracted from chicken embryonic brain tissue.

Application

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
ARPE cell lysate
Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
MABT12104053252293795

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