MABT25

Anti-Myosin 2 Antibody, clone 5B11.1

Name: Anti-Myosin 2 Antibody, clone 5B11.1
Brand: MM

clone 5B11.1, from mouse

Synonym(s):

Myosin-2, Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain Iia, MyHC-Iia, Myosin heavy chain, skeletal muscle, adult 2

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5B11.1, monoclonal

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG3κ

NCBI accession no.

NP_060004

UniProt accession no.

Q9UKX2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYH2(4620)

General description

Myosin is a hexameric protein consisting of two heavy chains including Myosin-2 (MYH2), two regulatory light chains, and two alkali light chains. The myosin-2 heavy chain interacts with the actin cytoskeleton and mediates muscle contractility in eukaryotic cells. Defects in myosin-2 result in inclusion body myopathy type 3.

~220 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to mouse Myosin 2.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Myosin 2 Antibody, clone 5B11.1 is validated for use in Western Blotting, IHC for the detection of Myosin 2.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 in L6 cell lysate (Myosin may be observed as a smear in some cell lysates).

Western Blot Analysis: A representative lot detected Myosin-2 in mouse heart cell cytosolic extracts.

Physical form

Format: Purified

Purified mouse monoclonal IgG3κ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human cardiac muscle tissue

Evaluated by Immunohistochemistry in human cardiac muscle tissue.

Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Myosin-2 in human cardiac muscle tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Myosin content of individual human muscle fibers isolated by laser capture microdissection.

Charles A Stuart et al.

American journal of physiology. Cell physiology, 310(5), C381-C389 (2015-12-18)

Muscle fiber composition correlates with insulin resistance, and exercise training can increase slow-twitch (type I) fibers and, thereby, mitigate diabetes risk. Human skeletal muscle is made up of three distinct fiber types, but muscle contains many more isoforms of myosin

Global Trade Item Number

SKU	GTIN
MABT25	04053252648380

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