MABT334
Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12
clone ZZ36-12, from mouse, purified by affinity chromatography
Synonym(s):
WASF2, WAVE-2, Wiskott-Aldrich syndrome protein family member 2, WASP family protein member 2, Protein WAVE-2, Verprolin homology domain-containing protein 2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
ZZ36-12, monoclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WASF2(10163)
General description
Wiskott-Aldrich Syndrome Protein (WASP)-family Protein Member 2 (known as WASF2/WAVE-2) has a very wide distribution with strong expression in peripheral blood leukocytes. WASF2/WAVE-2 is involved in signaling RTKs and small GTPases. In general WAVE family of proteins have a general function for regulating the actin cytoskeleton in various tissues.
~65 kDa observed.
May run at ~65-80 kDa, which is higher than calculated molecular weight of 54 kDa, due to the proline rich residues in the sequence (Yan, C., et al. (2003) EMBO J. 22(14): 3602–3612).
May run at ~65-80 kDa, which is higher than calculated molecular weight of 54 kDa, due to the proline rich residues in the sequence (Yan, C., et al. (2003) EMBO J. 22(14): 3602–3612).
Immunogen
Epitope: C-terminus
His-tagged recombinant protein corresponding to the C-terminus of Human WASF2/WAVE-2.
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-WASF2/WAVE-2 Antibody, clone ZZ36-12 is validated for use in Western Blotting and Immunocytochemistry for the detection of WASF2/WAVE-2.
Western Blotting Analysis: 1 µg/mL of a representative lot of this antibody detected WASF2/WAVE-2 in 10 µg of K562 cell lysate.
Western Blotting Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cell lysate +/- RNAi.
Immunocytochemistry Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cells.
Western Blotting Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cell lysate +/- RNAi.
Immunocytochemistry Analysis: A representative lot of this antibody detected WASF2/WAVE-2 in HeLa cells.
Physical form
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WASF2/WAVE-2 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected WASF2/WAVE-2 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
Certificates of Analysis (COA)
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