MABT538
Anti-Lamin A/C Antibody, clone 2E8.2
clone 2E8.2, from mouse
Synonym(s):
Prelamin-A/C, Lamin-A/C, Renal carcinoma antigen NY-REN-32
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About This Item
eCl@ss:
32160702
UNSPSC Code:
12352203
NACRES:
NA.46
Product Name
Anti-Lamin A/C Antibody, clone 2E8.2, clone 2E8.2, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2E8.2, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... LMNA(4000)
Analysis Note
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected lamin A/C in 10 µg of A431 cell lysate.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained nuclear membrane in human cerebellum, kidney, and pancreas tissue sections.
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Cat. No. MABT538 is validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of lamin A/C.
Biochem/physiol Actions
Clone 2E8.2 immunostained nuclear membrane in human tissue sections.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Lamin-A is a key component of the nuclear lamina, a protein network underlying the inner membrane that determines nuclear shape and size. The same gene (Lmna, Dhe, or Lamn1 in murine species; Gene ID 16905) that encodes lamin-A also codes for the alternatively spliced prelamin-C isoforms (UniProt P48678-2 and P48678-3). Murine lamin-A (UniProt P48678-1) is initially produced as a 665-amino acid prelamin A, which is further processed posttranslationally by four enzymatic steps to yield the mature Lamin-A. Farnesylation of Cys662, endoproteolytic cleavage of the last three amino acids (a.a. 663-665), carboxyl methylation of Cys662, and the final endoproteolytic removal of the remaining propeptide sequence (a.a. 648-662), including the farnesylated Cys662, catalyzed by the ER membrane zinc metalloproteinase ZMPSTE24.
~70/65 kDa observed. 74.14/73.81/72.22 kDa (perpro-/pre-/mature Lamin A) and 65.14 kDa (Lamin C) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
GST-tagged recombinant fragment within the C-terminal half of human Lamin A.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: MAB3211
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Luke W Koblan et al.
Nature, 589(7843), 608-614 (2021-01-08)
Hutchinson-Gilford progeria syndrome (HGPS or progeria) is typically caused by a dominant-negative C•G-to-T•A mutation (c.1824 C>T; p.G608G) in LMNA, the gene that encodes nuclear lamin A. This mutation causes RNA mis-splicing that produces progerin, a toxic protein that induces rapid
Zhuo Zhou et al.
Signal transduction and targeted therapy, 6(1), 382-382 (2021-11-05)
The global coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a positive-sense RNA virus. How the host immune system senses and responds to SARS-CoV-2 infection remain largely unresolved. Here, we report that SARS-CoV-2
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABT538 | 04054839114489 |
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