Anti-Lamin A/C Antibody, clone 2E8.2 Antibody, Alexa Fluor^™ 488 Conjugate

74.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Lamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (gene ID: 4000) in human. Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Proteolytic cleavage of 18 residues from the C-terminal of prelamin-A/C results in the production of lamin-A/C. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif, ZMPSTE24/FACE1 mediated cleavage of the last three amino acids, methylation of the C-terminal cysteine and endoproteolytic removal of the last 15 C-terminal amino acids. Farnesylation of prelamin-A/C also facilitates nuclear envelope targeting. Lamin A and C are present in equal amounts in the lamina of mammals. Lamin plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin C differs from canonical form A in the following manner: amino acids 567-572 are changed from GSHCSS to VSGSRR and form C does not amino acids 573-664 seen in canonical form A.

GST/His-tagged recombinant fragment corresponding to 109 amino acids from the C-terminal half of human Lamin A/C.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lamin A/C in A431 cells.

The unconjugated antibody (Cat. No. MABT538) is shown to be suitable also for Flow Cytometry, Immunohistochemistry, and Immunoprecipitation applications.

Research Category
Cell Structure

This mouse monoclonal Anti-Lamin A/C Antibody, clone 2E8.2, Alexa Fluor^™ 488 Conjugate, Cat. No. MABT538-AF488 is used for the detection of Lamin A/C by Immunocytochemistry.

Clone clone 2E8.2 specifically recognizes Lamin A/C in HeLa cells. This antibody has been shown to bind to an epitope between amino acids 464-572 in the C-terminal half.

Protein G purified

Purified mouse monoclonal antibody in PBS with 1.5% BSA and 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Lamin A/C in HeLa cells.

Concentration: Please refer to lot specific datasheet.

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