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Merck

MHSTCMAG-70K

MILLIPLEX® Mouse High Sensitivity T Cell Panel

Configurable Mouse High Sensitivity T Cell 18-Plex Panel

Synonym(s):

Luminex® Mouse Immunology Panel, Millipore Mouse Immunology Panel, Mouse Immunology Protein Multiplex Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Product Name

MILLIPLEX® Mouse High Sensitivity T Cell Panel, Configurable Mouse High Sensitivity T Cell 18-Plex Panel

species reactivity

mouse

manufacturer/tradename

Milliplex®

Quality Level

200

packaging

1 ea of

assay range

accuracy: 82-102%
sensitivity: 0.26-11.14 pg/mL
standard curve range: 0.10-400 pg/mL
(IL-4)
standard curve range: 0.49-2,000 pg/mL
(IFNγ)
standard curve range: 0.49-2,000 pg/mL
(IL-17A)
standard curve range: 0.49-2,000 pg/mL
(IL-7)
standard curve range: 0.49-2,000 pg/mL
(TNFα)
standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1)
standard curve range: 0.98-4,000 pg/mL
(IL-2)
standard curve range: 0.98-4,000 pg/mL
(IL-5)
standard curve range: 0.98-4,000 pg/mL
(IL-6)
standard curve range: 1.34-5,500 pg/mL
(IL-10)
standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70))
standard curve range: 3.17-13,000 pg/mL
(LIX)
standard curve range: 3.42-14.000 pg/mL
(IL-13)
standard curve range: 3.91-16,000 pg/mL
(IL-1α)
standard curve range: 3.91-16,000 pg/mL
(IL-1β)
standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2)
standard curve range: 6.1-25,000 pg/mL
(GM-CSF)
standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

input

cell culture supernatant(s)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Other Notes

Sensitivity: Please see protocol for sensitivity of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 18 analytes in mouse serum, plasma, and cell culture supernatants.

Analytes included: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

High Sensitivity Detection: Detects picogram-level cytokine concentrations, ideal for studying subtle immune responses in low-inflammation models.

Broad Analyte Coverage: Measures 18 key cytokines including IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12(p70), IL-13, IL-17A, IFNγ, TNFα, GM-CSF, MCP-1, MIP-2, KC, LIX, and IL-7.

Verified for In Vitro and In Vivo Models: Proven performance in stimulated PBMC cultures and mouse models of aging, obesity, and inflammation.

High Precision and Accuracy: Intra-assay CV <10%, inter-assay CV <15%, and accuracy between 82–102% across analytes.

Reveal Hidden Immune Signals: Ideal for detecting low-level cytokine expression that standard assays may miss—critical for understanding early or chronic immune responses.

General description

Mouse T cell cytokines are critical signaling molecules produced by T cells that play essential roles in the immune response. They facilitate communication between immune cells, influencing the activation, proliferation, and differentiation of various immune cell types. Key cytokines include interleukins (such as IL-2 and IL-4) and interferons, which help orchestrate both innate and adaptive immune responses in mice.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sha-Sha Yi et al.
Frontiers in psychiatry, 13, 864481-864481 (2022-05-17)
Increasing evidence indicates that inflammatory responses may influence brain neurochemical pathways, inducing depressive-like behaviors. Ultrasound stimulation (US) is a promising non-invasive treatment for neuropsychiatric diseases. We investigated whether US can suppress inflammation and improve depressive-like behaviors. Mice were intraperitoneally injected
Irene Martínez de Toda et al.
International journal of molecular sciences, 18(7) (2017-07-25)
The decrease in the proliferative response of lymphocytes is one of the most evident among the age-related changes of the immune system. This has been linked to a higher risk of mortality in both humans and experimental animals. However, long-lived
Robert A Kazmierczak et al.
Oncotarget, 11(44), 3943-3958 (2020-11-21)
Conventional cancer chemotherapies are not fully efficacious and do not target tumors, leading to significant treatment-related morbidities. A number of genetically attenuated cancer-targeting bacteria are being developed to safely target tumors in vivo. Here we report the toxicological, tumor-targeting, and
David K Harrison et al.
Transplantation, 105(3), 540-549 (2020-09-06)
Organ transplantation is life-saving and continued investigations into immunologic mechanisms that drive organ rejection are needed to improve immunosuppression therapies and prevent graft failure. DNA-dependent protein kinase catalytic subunit, DNA dependent-protein kinase catalytic subunit (DNA-PKcs), is a critical component of
Sébastien Le Garf et al.
International journal of molecular sciences, 20(20) (2019-10-23)
Regular aerobic exercise, independently of weight loss, improves metabolic and anti-inflammatory states, and can be regarded as beneficial in counteracting obesity-induced low-grade inflammation. However, it is still unknown how exercise alters immunometabolism in a context of dietary changes. Agonists of
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Related Content

Evaluation of MILLIPLEX® Mouse High Sensitivity T Cell Panel

The MILLIPLEX® Mouse High Sensitivity T Cell Multiplex Panel is a powerful tool for cytokine profiling in biological samples. Uncover details on how this panel was evaluated for studying low-level cytokine expression and also quantifying multiple cytokine secretion levels simultaneously.

Protocol- MHSTCMAG-70K|MHSTCMAG-70KPMX|MHSTCMAG-70KPXBK
Quality Control Range Sheet- HSTC-108 and HSTC-208
Quality Control Ranges: MILLIPLEX® MAP Mouse High Sensitivity T Cell Magnetic Bead Panel

Global Trade Item Number

SKUGTIN
MHSTCMAG-70K-09C04054839442308
MHSTCMAG-70K-1004054839221309
MHSTCMAG-70K-1304054839221330
MHSTCMAG-70K-04C04054839442254
MHSTCMAG-70K-15C04054839442360
MHSTCMAG-70K-08C04054839442292
MHSTCMAG-70K-10C04054839442315
MHSTCMAG-70K-0104054839221217
MHSTCMAG-70K-0704054839221279
MHSTCMAG-70K-1704054839221378
MHSTCMAG-70K-0804054839221286
MHSTCMAG-70K-1504054839221354
MHSTCMAG-70K-16C04054839442377
MHSTCMAG-70K-1204054839221323
MHSTCMAG-70K-11C04054839442322
MHSTCMAG-70K-03C04054839442247
MHSTCMAG-70K-0304054839221231
MHSTCMAG-70K-05C04054839442261
MHSTCMAG-70K-07C04054839442285
MHSTCMAG-70K-13C04054839442346
MHSTCMAG-70K-0404054839221248
MHSTCMAG-70K-17C04054839442391
MHSTCMAG-70K-0904054839221293
MHSTCMAG-70K-1404054839221347

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