Key Documents
NB37
Anti-Golgi Apparatus Mouse mAb (AE-6)
liquid, clone AE-6, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
Quality Level
antibody product type
primary antibodies
clone
AE-6
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human
should not react with
rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells. Recognizes the golgi apparatus protein.
Recognizes the Golgi apparatus in A431 and HeLa cells.
Immunogen
Human
the Golgi fraction of human cells
Application
Immunoblotting (2.5 µg/ml)
Immunofluorescence (1 µg/ml)
Immunofluorescence (1 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Positive Control
A-431 or HeLa cells
A-431 or HeLa cells
Other Notes
Antibody should be titrated for optimal results in individual systems.
Gomord, V., et al. 1999. Biochimie81, 607.
Burkhardt, J.K. 1998. Biochim. Biophys. Acta1404, 113.
Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta1404, 139.
Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol.10, 477.
Keenan, T.W., 1998. Histochem. Cell Biol.109, 505.
La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta1404, 195.
Munro, S., 1998. Trends Cell Biol.8, 11.
Pelham, H. R., 1998. Trends Cell Biol.8, 45.
Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol.10, 4993.
Burkhardt, J.K. 1998. Biochim. Biophys. Acta1404, 113.
Cabrera-Poch, N., et al. 1998. Biochim. Biophys. Acta1404, 139.
Kaiser, C., and Ferro-Novick, S., 1998. Curr Opin Cell Biol.10, 477.
Keenan, T.W., 1998. Histochem. Cell Biol.109, 505.
La Borgne, R., and Hoflack, B., 1998. Biochim Biophys Acta1404, 195.
Munro, S., 1998. Trends Cell Biol.8, 11.
Pelham, H. R., 1998. Trends Cell Biol.8, 45.
Warren, G., and Malhotra, V., 1998. Curr. Opin. Cell Biol.10, 4993.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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R Le Borgne et al.
Biochimica et biophysica acta, 1404(1-2), 195-209 (1998-08-26)
The trans-Golgi network (TGN) is the last station of the secretory pathway where soluble and membrane proteins are sorted for subsequent transport to endocytic compartments. This pathway is primarily followed by two distinct but related mannose 6-phosphate receptors which exhibit
Biochemistry of the Golgi apparatus.
T W Keenan
Histochemistry and cell biology, 109(5-6), 505-516 (1998-07-29)
V Gomord et al.
Biochimie, 81(6), 607-618 (1999-08-05)
Protein transport along the secretory pathway is supported by a noria of vesicles that bud and fuse, load and unload their cargo from one compartment into the other. However, despite this constant flow-through of proteins and lipids the various compartments
C Kaiser et al.
Current opinion in cell biology, 10(4), 477-482 (1998-08-28)
Two crucial aspects of transport vesicle function have recently been reconstituted using purified proteins and chemically defined lipid bilayers. The reconstituted steps are the assembly of a polymeric protein coat on the cytosolic surface of the membrane, and bilayer fusion
H R Pelham
Trends in cell biology, 8(1), 45-49 (1998-08-08)
Electron micrographs of the Golgi apparatus typically show a series of flat cisternae stacked together, surrounded by numerous vesicles and tubules. Palade and colleagues established in the 1960s that secretory proteins pass through this morphologically complex organelle as they travel
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