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OP07L

Sigma-Aldrich

Anti-v-Src (Ab-1) Mouse mAb (327)

lyophilized, clone 327, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

antibody product type

primary antibodies

clone

327, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

General description

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of v-Src (Ab-1).

Immunogen

Epitope: within the SH3 domain of v-Src and c-Src
purified v-Src

Application

Frozen Sections (not recommended)

Immunoblotting (2.5 µg/ml, see application references)

Immunofluorescence (not recommended)

Immunoprecipitation (1 µg/sample or use Cat. No. OP07A; see application references)

Paraffin Sections (not recommended)

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Preparation Note

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, refrigerate (4°C) with 0.1% azide for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid repeated freeze/thaw cycles of solutions. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h.

Analysis Note

Positive Control
NIH/3T3 cells

Other Notes

Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src kinase(see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

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