OP104L

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

Name: Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)
Brand: MM

lyophilized, clone PAb1802, Calbiochem^®

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802), lyophilized, clone PAb1802, Calbiochem^®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb1802, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

dilution

(Immunoblotting (2.5 µg/mL, chemiluminescence,
Immunoprecipitation (1 µg/reaction,
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended))

isotype

IgG1

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TP53(7157)

Analysis Note

Negative Control
SK-OV-3 cells

Positive Control
A431 or SK-BR-3 cells

Application

Immunoblotting (2.5 µg/ml, chemiluminescence, see application references)

Immunoprecipitation (1 µg/reaction, see comments, see application references)

Frozen Sections (not recommended)

Immunofluorescence (not recommended)

Paraffin Sections (not recommended)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.

Recognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.

This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).

Immunogen

Epitope: within amino acids 306-393 of human p53

Human

recombinant, human p53 protein

Other Notes

Levine, A.J. 1997. Cell88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol.17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J.15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA92, 9455.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Clarke, A.R., et al. 1994. Oncogene9, 1767.
Cho, Y., et al. 1994. Science265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.

This antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Preparation Note

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chronic hypoxemia in late gestation decreases cardiomyocyte number but does not change expression of hypoxia-responsive genes.

Kimberley J Botting et al.

Journal of the American Heart Association, 3(4) (2014-08-03)

Placental insufficiency is the leading cause of intrauterine growth restriction in the developed world and results in chronic hypoxemia in the fetus. Oxygen is essential for fetal heart development, but a hypoxemic environment in utero can permanently alter development of