Skip to Content
Merck

OP120

Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5)

liquid, clone H3.105.5, Calbiochem®

Synonym(s):

Anti-HER3, Anti-ErbB3, Anti-Erythroblastosis Virus

Sign In to View Organizational & Contract Pricing

Select a Size

All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

H3.105.5, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ERBB3(2065)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma NSO cells (see application references). Recognizes the ~185 kDa c-Erb B3/HER-3 protein.
Recognizes the c-ErbB3/HER 3 protein in T47D cells and breast and prostate carcinoma tissue.
This Anti-c-ErbB3 (Ab-5) Mouse mAb (H3.105.5) is validated for use in FC, Frozen Sections, Immunoblotting, IP, Neutralization Studies, Paraffin Sections for the detection of c-ErbB3 (Ab-5).

Immunogen

Human
recombinant, human ErbB3 extracellular domain

Application

Flow Cytometry (see comments)

Frozen Sections (not recommended)

Immunoblotting (not recommended)

Immunofluorescence (see comments)

Immunoprecipitation (10 µl/mg protein lysate)

Neutralization Studies (see application references)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.4.

Preparation Note

For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.

Analysis Note

Positive Control
T47D cells or breast or prostate carcinoma

Other Notes

Chen, X., et al. 1996. J. Biol. Chem.271, 7620.
Carraway, K.L., et al. 1994. J. Biol. Chem.269, 14303.
Ciardiello, F., et al. 1991. Proc. Natl. Acad. Sci. USA88, 7792.
Plowman, G.D., et al. 1990. Proc. Natl. Acad. Sci. USA87, 4905.
Kraus, J.H., et al. 1989. Proc. Natl. Acad. Sci. USA86, 9193.
Shoyab, M., et al. 1989. Science243, 1074.
Kawakami, T., et al. 1986. Mol. Cell. Biol.6, 4195.
Does not cross-react with c ErbB1 (EGFR), c-ErbB2/HER-2/neu or c-ErbB4/HER-4. c-ErbB3/HER-3 is overexpressed in a variety of tumors (e.g., breast, stomach, pancreas and colon). This antibody is particularly well suited for immunoprecipitating the unoccupied receptor. Reported to block neuregulin-1 β growth and signalling effects in ovarian cancer cell lines. This antibody has also been reported to work for flow cytometry and immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zsófia Pénzváltó et al.
Journal of mammary gland biology and neoplasia, 24(2), 149-162 (2019-02-28)
In order to develop a practical model of breast cancer, with in vitro and syngeneic, immune-intact, in vivo growth capacity, we established a primary cell line derived from a mammary carcinoma in the transgenic FVB/N-Tg(MMTV-ErbB2*)NDL2-5Mul mouse, referred to as "NDLUCD". The

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service