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Merck

OP134

Anti-IKKβ Mouse mAb (10AG2)

liquid, clone 10AG2, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

10AG2, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... IKBKB(3551)

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa IKKβ protein.
Recognizes the ~87 kDa IKKβ protein in Daudi cells.
This Anti-IKKβ Mouse mAb (10AG2) is validated for use in Immunoblotting for the detection of IKKβ.

Immunogen

Human
a recombinant protein consisting of full-length human IKKβ fused to a His•Tag sequence

Application


Immunoblotting (1-4 g/ml, chemiluminescence)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.2% gelatin, pH 7.4.

Analysis Note

Positive Control
Daudi cells

Other Notes

Antibody should be titrated for optimal results in individual systems.
Delhase, M., et al. 1999. Science284, 309.
Li. Q., et al. 1999. Science284, 321.
Didonato, et al. 1997. Nature388, 548.
Lee, F.S., et al. 1997. Cell88, 213.
Malinin, N.L., et al. 1997. Nature385, 540.
Mercurio, F., et al. 1997. Science278, 860.
Regnier, C.H., et al. 1997. Cell90, 373.
Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA94, 11758.
Verma, I.M., et al. 1995. Genes. Dev.9, 2723.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jonathan M Starkey et al.
Diabetes, 55(5), 1252-1259 (2006-04-29)
Evidence of diabetes-induced nuclear factor-kappaB (NF-kappaB) activation has been provided with DNA binding assays or nuclear localization with immunohistochemistry, but few studies have explored mechanisms involved. We examined effects of diabetes on proteins comprising NF-kappaB canonical and noncanonical activation pathways
Tamara P Martin et al.
Cellular signalling, 51, 166-175 (2018-07-31)
Calcium/calmodulin dependent protein kinase IIδ (CaMKIIδ) acts as a molecular switch regulating cardiovascular Ca2+ handling and contractility in health and disease. Activation of CaMKIIδ is also known to regulate cardiovascular inflammation and is reported to be required for pro-inflammatory NF-κB

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