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OP66

Sigma-Aldrich

Anti-Rb (Ab-5) Mouse mAb (LM95.1)

liquid, clone LM95.1, Calbiochem®

Synonym(s):

Anti-Retinoblastoma Associated Protein, Anti-pp105

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

LM95.1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RB1(5925)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~105 kDa Rb protein.
Recognizes the ~105 kDa Rb protein. Sold under license of U.S. Patent 5,853,988 and corresponding patents.
This Anti-Rb (Ab-5) Mouse mAb (LM95.1) is validated for use in Immunoblotting, Paraffin Sections for the detection of Rb (Ab-5).

Immunogen

Epitope: within a 56 kDa C-terminal fragment
Human
recombinant, human Rb

Application

Immunoblotting (1 µg/ml; see application references)

Paraffin Sections (3 µg/ml, see comments and application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
Saos cells
Positive Control
ML-1 cells or normal colon tissue

Other Notes

Dyson, N., et al. 1992. J. Virol.66, 4606.
Dyson, N., et al. 1990. J. Virol.64, 1353.
Horowitz, J.M., et al. 1989. Science243, 937.
Whyte, P., et al. 1988. Nature334, 124.
Klein, G. 1987. Science238, 1539.
Lee, W.H., et al. 1987. Science235, 1394.
Lee, W.H., et al. 1987. Nature329, 642.
Knudson, A.G. 1971. Proc. Natl. Acad. Sci. USA68, 820.
Pre-treatment with heat or pressure cooker may improve staining of some tissue specimens; this should be determined for each specimen. Antibody should be titrated for optimal results in individual systems.

Legal Information

Sold under license of U.S. Patent 5,853,988 and corresponding patents.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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