biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FGF2(2247)
General description
Anti-FGF, Basic (Ab-2) (40-63), rabbit polyclonal, recognizes the ~18 kDa bFGF protein in fetal liver and kidney. It is validated for Western blotting an immunohistochemistry (paraffin sections).
Purified rabbit polyclonal antibody (see application references). Recognizes the ~18 kDa bFGF protein.
Recognizes the ~18 kDa bFGF protein in fetal liver and kidney tissue.
Immunogen
Human
a synthetic peptide (GFFLRIHPDGRVDGVREKSDPHIK) corresponding to amino acids 40-63 of human bFGF
Application
Immunoblotting (1-5 µg/ml)
Paraffin Sections (1-5 µg/ml, no pre-treatment required)
Paraffin Sections (1-5 µg/ml, no pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM PBS, 50% glycerol.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Fetal liver or kidney tissue
Fetal liver or kidney tissue
Other Notes
Antibody should be titrated for optimal results in individual systems.
Burton, P.B.J., et al. 1993. Int. J. Exp. Path.74, 87.
Fiege, J.J. and Baird, A. 1989. Proc. Natl. Acad. Sci.86, 3174.
Florkiewicz, R.Z. and Sommer, A. 1989. Proc. Natl. Acad. Sci.86, 3978.
Rifkin, D.B. and Moscatelli, D. 1989. J. Cell Biol.109, 1.
Baird, A., et al. 1988. Proc. Natl. Acad. Sci.85, 2324.
Chodak, G.W., et al. 1988. Cancer Res. 48, 2083.
Sommer, A., et al. 1987. Res. Comm.144, 543.
Abraham, J.A., et al.1986. EMBO J.5, 2523.
Esch, F., et al.1985. Proc. Natl. Acad. Sci.82, 6507.
Knighton, D., et al. 1977. Br. J. Cancer35, 347.
Fiege, J.J. and Baird, A. 1989. Proc. Natl. Acad. Sci.86, 3174.
Florkiewicz, R.Z. and Sommer, A. 1989. Proc. Natl. Acad. Sci.86, 3978.
Rifkin, D.B. and Moscatelli, D. 1989. J. Cell Biol.109, 1.
Baird, A., et al. 1988. Proc. Natl. Acad. Sci.85, 2324.
Chodak, G.W., et al. 1988. Cancer Res. 48, 2083.
Sommer, A., et al. 1987. Res. Comm.144, 543.
Abraham, J.A., et al.1986. EMBO J.5, 2523.
Esch, F., et al.1985. Proc. Natl. Acad. Sci.82, 6507.
Knighton, D., et al. 1977. Br. J. Cancer35, 347.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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