PC322L
Anti-VEGFR1 Goat pAb
lyophilized, Calbiochem®
Synonym(s):
Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor
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About This Item
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human
Application:
—
Citations:
1
biological source
goat
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FLT1(2321)
General description
This Anti-VEGFR1 Goat pAb is validated for use in Competition Studies, ELISA, Immunoblotting, Paraffin Sections for the detection of VEGFR1.
Immunoaffinity purified goat polyclonal antibody. Recognizes the VEGF receptor 1 protein.
Recognizes VEGF receptor 1.
Immunogen
Human
recombinant, human VEGFR1 (extracellular domain)/Fc chimera
Application
Competition Studies (see comments)
ELISA (0.5-1 µg/ml)
Immunoblotting (0.1-0.2 µg/ml)
Paraffin Sections (5-15 µg/ml)
ELISA (0.5-1 µg/ml)
Immunoblotting (0.1-0.2 µg/ml)
Paraffin Sections (5-15 µg/ml)
Physical form
Lyophilized from a sterile solution in PBS, 5% trehalose.
Preparation Note
Reconstitute the lyophilized antibody in 1 ml sterile PBS. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Endothelial cells
Endothelial cells
Other Notes
Soker, S., et al. 1998. Cell92, 735.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS.90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303.
Brown, L., et al. 1992. Kidney International42, 1457.
Kroll, J. and Waltenberger, J. 1997. J. Biol. Chem.272, 32521.
Kendall, R.L. and Thomas, K.A. 1993. PNAS.90, 10705.
Klagsbrun, M. and Soker, S. 1993. Current Biology3, 699.
Senger, D., et al. 1993. Cancer and Metastasis Rev.12, 303.
Brown, L., et al. 1992. Kidney International42, 1457.
This antibody has been selected for its ability to recognize human recombinant VEGFR-1 in immunoblotting and ELISA. The detection limit for human recombinant VEGFR-1 by immunoblotting is approximately 50 ng/lane under reducing and non-reducing conditions. The detection limit for human recombinant Flt-1 by ELISA is approximately 0.16 ng/well. At approximately 10 µg/ml this antibody will completely block the binding of rhPlGF (2 ng/ml) to immobilized rhVEGFR1/Fc (1 µg/ml; 100 µl/well). This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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