PC635

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

Name: PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Nibrin (pSer³⁴³) Rabbit pAb

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Product Name

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb, liquid, Calbiochem^®

biological source

rabbit

antibody form

diluted serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µL/500 µg cell extract))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer343)

Quality Level

100

Gene Information

human ... NBN(4683)

General description

Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.

This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

Immunogen

Human

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

Analysis Note

Positive Control
γ-irradiated lymphoblastoid cells

Application

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

Disclaimer

Toxicity: Standard Handling (A)

Other Notes

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.

This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

Physical form

Serum diluted in PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c