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Merck

PF125

BACE1, His•Tag®, Human, Recombinant, NSO Cells

Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Supplied as a mixture of ~68-70 kDa proenzyme and ~65-67 kDa mature enzyme.

Synonym(s):

Human β-Secretase

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About This Item

UNSPSC Code:
12352202

Product Name

BACE1, His•Tag®, Human, Recombinant, NSO Cells, Recombinant, human BACE-1 (amino acids 1-460) fused at the C-terminus to a His•Tag sequence and expressed in NSO cells. Supplied as a mixture of ~68-70 kDa proenzyme and ~65-67 kDa mature enzyme.

recombinant

expressed in NSO cells

assay

≥90% (SDS-PAGE)

form

lyophilized

specific activity

≥1 pmol/min-μg protein

does not contain

preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1 EU/μg Endotoxin (EU/μg BACE-1)

solubility

water: 500 μg/mL

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Disclaimer

Toxicity: Standard Handling (A)

General description

as measured by the cleavage of 10 μM MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(DNP)-Arg-Arg-NH2 fluorescent substrate using 100 ng BACE-1 in 0.1 M sodium acetate (pH 4.0) at room temperature

Other Notes

Ermolieff, J. et al. 2000. Biochemistry39, 12450.
Lin, X. et al. 2000. Proc. Natl. Acad. Sci. USA97, 1456.
Sinha, S. et al. 1999. Nature402, 537.
Vassar, R. et al. 1999. Science286, 735.
Yan, R. et al. 1999. Nature402, 533.

Physical form

Lyophilized from sterile-filtered PBS.

Preparation Note

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 4 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Amanda Gonzales et al.
Journal of neuroscience methods, 202(1), 70-76 (2011-09-06)
The aspartyl protease BACE1 is the rate limiting enzyme in the synthesis of amyloid beta, which accumulation in the human brain is a hallmark of Alzheimer's disease (AD). BACE1 has been proposed as a surrogate marker of AD; however, very

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