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Merck

PK1008

PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹)

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr751)

Gene Information

human ... PDGFRB(5159)

General description

Rabbit polyclonal antibody purified by protein A and immunoaffinity chromatography. Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751.
Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells.
This PhosphoDetect Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb is validated for use in FC, Immunoblotting, IP, Paraffin Sections for the detection of PDGFRβ(pTyr⁷⁵¹).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH

Application




Flow Cytometry (1:200)
Immunoblotting (1:1000)
Immunoprecipitation (1:50)
Paraffin Sections (1:50, heat pre-treatment required; see comments)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Preparation Note

Do not aliquot.

Analysis Note

Positive Control
Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells

Other Notes

Betsholtz, C., et al. 2001. Bioessays23, 494.
Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res.80, 1.
Ramalingam, K., et al. 1995. Bioorg. Med. Chem.3, 1263.
Panyotou, G. et al. 1992. EMBO J.11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res.266, 39.
Deuel, T.F., et al. 1988. Biofactors1, 213.
For staining paraffin sections bring slides to a boil in 1 mM EDTA, pH 8.0 and continue to heat at sub-boiling temperature for 15 min; no cooling is necessary. Wash paraffin sections with 1X TBS, 0.1% Tween®-20 detergent. For immunoblotting membranes should be incubated with antibody diluted in 1X TBS, 5% BSA, 0.1% Tween-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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