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Merck

ST1622

Anti-LEPRE1 Mouse mAb (3C7)

liquid, clone 3C7, Calbiochem®

Synonym(s):

Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Product Name

Anti-LEPRE1 Mouse mAb (3C7), liquid, clone 3C7, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

3C7, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (6 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Positive Control
HeLa cells, Human esophagus tissue

Application




ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (6 g/ml)

Disclaimer

Toxicity: Regulatory Review (Z)

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.

Immunogen

A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein

Other Notes

Choi, JW, et al. 2009. PLoS Genet.5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet.39, 359.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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