biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C3, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GRK6(2870)
General description
Anti-GRK6, mouse monoclonal, clone 2C3, recognizes the ~65-70 kDa GRK6 protein in HeLa and Jurkat cells. It is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~65-70 kDa GRK6 protein.
Recognizes the ~65-70 kDa GRK6 protein in HeLa and Jurkat cells.
Immunogen
A recombinant polypeptide corresponding to amino acids of 71-170 human GRK6, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Jurkat cells
HeLa cells, Jurkat cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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