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Merck

DCTP-RO

Roche

dCTP

99% (dNTP), <0.9% (dNDP), solution, 100 mM

Synonym(s):

2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na2

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About This Item

Empirical Formula (Hill Notation):
C9H14N3Na2O13P3
CAS Number:
102783-51-7
Molecular Weight:
511.12
UNSPSC Code:
41106305
PubChem Substance ID:
329798916
MDL number:
MFCD00084683
NACRES:
NA.54

Product Name

dCTP, 99% (dNTP), <0.9% (dNDP), solution, 100 mM

InChI

1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1

SMILES string

[Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2

InChI key

ABWVCNMFYVEBIB-CDNBRZBRSA-L

description

PCR Grade, sodium salt

assay

99% (dNTP)

form

solution

usage

sufficient for 125,000 standard reactions (03732690001)
 sufficient for 31,250 standard reactions (11969021001)
 sufficient for 6,250 standard reactions (11934520001)

packaging

pkg of 1,250 μL (11969021001 [100 mM])
pkg of 4 × 1,250 μL (03732690001 [4x100 mM])
pkg of 250 μL (11934520001 [100 mM])

manufacturer/tradename

Roche

concentration

<0.9% (dNDP)
100 mM

storage temp.

−20°C

Quality Level

100

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Analysis Note

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Other Notes

For general laboratory use.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
11621420
54587621
53879422
53879421
49206620

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Alix T Coste et al.
Eukaryotic cell, 3(6), 1639-1652 (2004-12-14)
The ABC transporter genes CDR1 and CDR2 can be upregulated in Candida albicans developing resistance to azoles or can be upregulated by exposing cells transiently to drugs such as fluphenazine. The cis-acting drug-responsive element (DRE) present in the promoters of
Mahir Karababa et al.
Antimicrobial agents and chemotherapy, 48(8), 3064-3079 (2004-07-27)
Azole resistance in Candida albicans can be due to upregulation of multidrug transporters belonging to ABC (ATP-binding cassette) transporters (CDR1 and CDR2) or major facilitators (CaMDR1). Upregulation of these genes can also be achieved by exposure to fluphenazine, resulting in
Xiangang Zong et al.
Pflugers Archiv : European journal of physiology, 458(5), 891-899 (2009-06-27)
Second messenger-induced Ca(2+)-release from intracellular stores plays a key role in a multitude of physiological processes. In addition to 1,4,5-inositol trisphosphate (IP(3)), Ca(2+), and cyclic ADP ribose (cADPR) that trigger Ca(2+)-release from the endoplasmatic reticulum (ER), nicotinic acid adenine dinucleotide

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