D2532

Denhardt′s Solution 50x

Name: Denhardt′s Solution 50x
Brand: SIGMA

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Product Name

Denhardt′s Solution 50x, for Northern and Southern blotting, solution

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

Quality Level

300

Related Categories

Biochemicals

Biological Buffers

Chemistry & Biochemicals

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Gel Electrophoresis Equipment and Systems

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000484704

0000411631

0000375543

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Distribution of glucocorticoid and mineralocorticoid receptors and 11beta-hydroxysteroid dehydrogenases in human and rat ocular tissues.

J Stokes et al.

Investigative ophthalmology & visual science, 41(7), 1629-1638 (2000-06-14)

The administration of glucocorticoids as topical or systemic medications may lead to the development of ocular hypertension through the induction of morphologic and biochemical changes in the trabecular meshwork leading to a reduction in the facility of aqueous outflow. Glucocorticoids

Galanin receptor 3--a potential target for acute pancreatitis therapy.

Barreto SG

Neurogastroenterology and Motility, 23(3), 23(3)-23(3) (2011)

Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections.

Gregor Obernosterer et al.

Nature protocols, 2(6), 1508-1514 (2007-06-16)

Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in

Tenascin in bone morphogenesis: expression by osteoblasts and cell type-specific expression of splice variants.

E J Mackie et al.

Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)

The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.

Expression of a cryptic secondary sigma factor gene unveils natural competence for DNA transformation in Staphylococcus aureus.

Kazuya Morikawa et al.

PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)

It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for

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