N1531

Denaturation Solution

Name: Denaturation Solution
Brand: SIGMA

for Northern and Southern blotting

Synonym(s):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Product Name

Denaturation Solution, for Northern and Southern blotting

grade

Molecular Biology

form

solution

storage temp.

room temp

Quality Level

200

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Other Notes

0.5 N NaOH, 1.5 M NaCl

The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.

pictograms

GHS05

signalword

Danger

hcodes

H290,H314

pcodes

P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000489811

0000445851

0000438535

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The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling.

José Manuel Pérez-Pérez et al.

Plant physiology, 134(1), 101-117 (2004-01-20)

The dwarf ucu (ultracurvata) mutants of Arabidopsis display vegetative leaves that are spirally rolled downwards and show reduced expansion along the longitudinal axis. We have previously determined that the UCU1 gene encodes a SHAGGY/GSK3-like kinase that participates in the signaling

Sambrook, J., et al.

Molecular Cloning: A Laboratory Manual, 9-9 (1989)

Ausubel, F.M., et al.

Short Protocols in Molecular Biology (1995)

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.