N6019
Neutralizing Buffer
for Northern and Southern blotting, powder
Synonym(s):
Neutralizing Buffer for Blotting Techniques
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Product Name
Neutralizing Buffer, for Northern and Southern blotting, powder
grade
Molecular Biology
form
powder
technique(s)
Southern blotting: suitable
foreign activity
DNase, RNase, none detected
storage temp.
room temp
Quality Level
Application
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
General description
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
Preparation Note
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
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