O3756

Orange G

Name: Orange G
Brand: SIGMA

for NA electrophoresis

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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All Photos(3)

About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₀N₂Na₂O₇S₂

CAS Number:

1936-15-8

Molecular Weight:

452.37

EC Number:

217-705-6

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

57654483

MDL number:

MFCD00012457

Colour Index Number:

16230

Beilstein/REAXYS Number:

4120705

Product Name

Orange G, for NA electrophoresis

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

grade

Molecular Biology

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL, clear, orange to red

suitability

suitable for gel electrophoresis

storage temp.

room temp

Quality Level

200

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Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

Application

Orange G has been used as a DNA gel loading dye.

Orange G is a tracking dye used in nucleic acid electrophoresis to track DNA front in agarose gels. Also used in SDS-capillary electrophoresis as a standard. Typically runs at 50bp in up to 2.5% agarose.

General description

Orange G is an azo dye that is acidic in nature. It is widely used as a tracking dye for nucleic acid gel electrophoresis to track DNA front in agarose gels. Typically runs at 50bp in up to 2.5% agarose.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Comprehensive Cytopathology (2014)

Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis.

A Guttman et al.

Journal of chromatography, 593(1-2), 297-303 (1992-02-28)

The influence of the primary structure (base composition) on the electrophoretic migration properties of single-stranded oligodeoxyribonucleotides in capillary polyacrylamide gel electrophoresis was investigated using homo- and heterooligomers under denaturing and non-denaturing conditions. Homooligodeoxyribonucleotides of equal chain lengths but of different

Degradation of azo dye Orange G in aqueous solutions by persulfate with ferrous ion

Xu XR and Li xZ

Separation and Purification Technology, 72(1), 105-111 (2010)

Differential salt fractionation of nuclei to analyze chromatin-associated proteins from cultured mammalian cells

Herrmann C, et al.

Bio-protocol, 7(6), 1986-1986 (2017)

Analysis of low-molecular-mass proteins in cerebrospinal fluid by sodium dodecyl sulfate capillary gel electrophoresis.

A Hiraoka et al.

Journal of chromatography. B, Biomedical sciences and applications, 697(1-2), 141-147 (1997-10-29)

Proteins of low molecular (M(r)) in human cerebrospinal fluid (CSF) were analyzed by capillary electrophoresis in a sodium dodecyl sulfate-containing polymer solution. Under the conditions employed, peaks of beta 2-microglobulin (beta MG) (M(r): 11,700), gamma-trace protein (12,300), myelin basic protein

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