R7020

Transcript RNA Markers 0.2-10 kb

Name: Transcript RNA Markers 0.2-10 kb
Brand: SIGMA

for RNA electrophoresis

Synonym(s):

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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About This Item

NACRES:

NA.25

UNSPSC Code:

41105337

Product Name

Transcript RNA Markers 0.2-10 kb, for RNA electrophoresis

grade

Molecular Biology

form

liquid

usage

25 uses

solubility

water: soluble

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

Quality Level

300

Related Categories

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Gel Electrophoresis Equipment and Systems

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides

For optimal resolution, the recommended agarose gel concentration is 1.0%.

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000406909

SLCQ8407

0000342041

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Barley lesion mimics, supersusceptible or highly resistant to leaf rust and net blotch.

Wright SA, et al.

Plant Pathology, 62, 982-992 (2013)

Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT.

Magda Dudek et al.

Nucleic acids research, 48(14), 7786-7800 (2020-06-26)

Marine flavobacteria possess dedicated Polysaccharide Utilization Loci (PULs) enabling efficient degradation of a variety of algal polysaccharides. The expression of these PULs is tightly controlled by the presence of the substrate, yet details on the regulatory mechanisms are still lacking.

Articles

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocols

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Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.