T9650

Tris Acetate-EDTA buffer

Name: Tris Acetate-EDTA buffer
Brand: SIGMA

10× concentrate, BioReagent, Molecular Biology, non-sterile; 0.2 μm filtered

Synonym(s):

TAE buffer

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All Photos(2)

About This Item

NACRES:

NA.25

PubChem Substance ID:

329826908

UNSPSC Code:

41105319

MDL number:

MFCD00236357

Product Name

Tris Acetate-EDTA buffer, 10× concentrate, BioReagent, Molecular Biology, non-sterile; 0.2 μm filtered

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

grade

Molecular Biology

sterility

non-sterile; 0.2 μm filtered

product line

BioReagent

form

solution

suitability

suitable for gel electrophoresis (after dilution to working concentration)

foreign activity

Protease, none detected
RNAse, none detected

Quality Level

200

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Related Categories

Biochemicals

Biological Buffers

Application

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

Other Notes

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

pictograms

GHS08

signalword

Warning

hcodes

H373

pcodes

P260 - P313 - P501

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000458827

0000446250

0000444846

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TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.