P7288

o-Phenylenediamine dihydrochloride

Name: <I>o</I>-Phenylenediamine dihydrochloride
Brand: SIGMA

chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

Select a Size

All Photos(2)

About This Item

Linear Formula:

C₆H₄(NH₂)₂·2HCl

CAS Number:

615-28-1

Molecular Weight:

181.06

NACRES:

NA.83

PubChem Substance ID:

24898837

UNSPSC Code:

12352204

EC Number:

210-418-7

MDL number:

MFCD00012966

Beilstein/REAXYS Number:

3912045

Product Name

o-Phenylenediamine dihydrochloride, tablet, 20 mg substrate per tablet

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Protein Biology

Proteins & Enzymes

Detection Substrates & Enzymes

Application

o-Phenylenediamine dihydrochloride has been used:

as a substrate for the eosinophil peroxidase (EPO) assay in bronchoalveolar lavage fluid (BALF)
in cell adhesion molecule-enzyme immunoassay (CAM-EIA) to analyze the amounts of E-selectin induced by test compounds (LPS or solutions and materials) on endothelial cells
in ultra-sensitive enzyme-linked immunosorbent assay (ELISA) to measure prolactin and luteinizing hormone (LH)

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302 + H332,H317,H319,H341,H351,H410

pcodes

P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay.

Ronald E Unger et al.

Biomaterials, 35(10), 3180-3187 (2014-01-25)

Endotoxin, also known as lipopolysaccharide (LPS) produced by bacteria can be present in any liquid or on any biomaterial even if the material is sterile. Endotoxin in mammals can cause fever, inflammation, cell and tissue damage and irreversible septic shock

Norovirus-binding proteins recovered from activated sludge micro-organisms with an affinity to a noroviral capsid peptide.

D Sano et al.

Journal of applied microbiology, 109(6), 1923-1928 (2010-08-26)

Transmission routes of noroviruses, leading aetiological agents of acute gastroenteritis, are rarely verified when outbreaks occur. Because the destination of norovirus particles being firmly captured by micro-organisms could be totally different from that of those particles moving freely, micro-organisms with

Elevated prolactin secretion during proestrus in mice: Absence of a defined surge.

Hollian R Phillipps et al.

Journal of neuroendocrinology, 34(6), e13129-e13129 (2022-05-02)

Throughout the reproductive cycle in rodents, prolactin levels are generally low. In some species, including rats, a prolactin surge occurs on proestrus with peak concentrations coinciding with the preovulatory luteinizing hormone (LH) surge. In mice, however, there are conflicting reports

Whole-blood model.

C A Ison

Methods in molecular medicine, 66, 317-329 (2001-01-01)

Neisseria meningitidis is an obligate human pathogen. When it interacts with the host, it can establish a commensal relationship or can, on a minority of occasions, invade and cause systemic disease. Protection against systemic disease, particularly for serogroup A and

Newly discovered anti-inflammatory properties of the benzamides and nicotinamides.

R W Pero et al.

Molecular and cellular biochemistry, 193(1-2), 119-125 (1999-05-20)

Our laboratory has concentrated on the possible regulation the benzamides and nicotinamides may have on the processes of DNA repair and apoptosis. Recent reports have suggested that both apoptosis and inflammation are regulated by the transcription factor NF-kappaB. We have

View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.