Skip to Content
Merck

66825-U

BIOshell™ A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

Protein C4 Reversed-Phase Chromatography Column

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
L × i.d.:
10 cm × 2.1 mm
Particle size:
3.4 μm
Matrix active group:
C4 (butyl) phase
Pore size:
400 Å
Matrix:
spherical silica particle platform
superficially porous particle

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

BIOshell™ A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

agency

suitable for USP L26

description

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

feature

endcapped

packaging

1 ea of

extent of labeling

0.4% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

15 m2/g

surface coverage

4.2 μmol/m2

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

3.4 μm

pore size

400 Å

operating pH range

2-9

separation technique

reversed phase

Quality Level

100

product line

BIOshell™

manufacturer/tradename

BIOshell™

Looking for similar products? Visit Product Comparison Guide

Related Categories

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

General description

BIOshell™ 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
USJUQ001673
USJUQ001671
USJUQ001670
USJUQ001669
USJUQ001668

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column

Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.

Biosimilar Comparability Workflow for Intact Mass Analysis

A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.

Middle Up Mass Analysis Using Trastuzumab as Model Protein

A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

See All

Protocols

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell A400 Protein C4

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

Faster Protein LC (FP2LC)

Larger porous shell particles in BIOshell™ columns improve efficiency in U/HPLC analysis of peptides and proteins.

Related Content

Large Molecule HPLC

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service